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KMID : 0848120210460010015
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International Journal of Oral Biology
2021 Volume.46 No. 1 p.15 ~ p.22
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Alpha-lipoic acid protects human dopaminergic neuronal cells against hydrogen peroxide-induced cell injury by inhibiting autophagy and apoptosis
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Kang Kyeong-Rok
Kim Jae-Sung
Kim Tae-Hyeon
Seo Jeong-Yeon
Lim Hyang-I
Park Jong-Hyun
Yang Kwang-Yeol
Yu Sun-Kyoung
Kim Heung-Joong
Kim Chun-Sung
Chun Hong-Sung
Lee Dong-Seol
Park Joo-Cheol
Kim Do-Kyung
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Abstract
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Alpha-lipoic acid (ALA) is a naturally occurring antioxidant and has been previously used to treat diabetes and cardiovascular disease. However, the autophagy effects of ALA against oxidative stress-induced dopaminergic neuronal cell injury remain unclear. The aim of this study was to investigate the role of ALA in autophagy and apoptosis against oxidative stress in the SH-SY5Y human dopaminergic neuronal cell line. We examined SH-SY5Y phenotypes using the 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide assay (cell viability/proliferation), 4′,6-diamidino-2-phenylindole dihydrochloride nuclear staining, Live/Dead cell assay, cellular reactive oxygen species (ROS) assay, immunoblotting, and immunocytochemistry. Our data showed ALA attenuated hydrogen peroxide (H2O2)-induced ROS generation and cell death. ALA effectively suppressed Bax up-regulation and Bcl-2 and BclxL down-regulation. Furthermore, ALA increased the expression of the antioxidant enzyme, heme oxygenase-1. Moreover, the expression of Beclin-1 and LC-3 autophagy biomarkers was decreased by ALA in our cell model. Combined, these data suggest ALA protects human dopaminergic neuronal cells against H2O2-induced cell injury by inhibiting autophagy and apoptosis.
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KEYWORD
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Alpha-lipoic acid, Autophagy, Hydrogen peroxide, Neuronal cell
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